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	Optofluidic techniques for directed evolution of enzymes Pilát, Zdeněk ; Ježek, Jan ; Samek, Ota ; Zemánek, Pavel ; Buryška, T. ; Damborský, J. ; Prokop, Z. Enzymes are highly versatile and ubiquitous biological catalysts. They can greatly accelerate\nlarge variety of reactions, while ensuring appropriate catalytic activity and high selectivity.\nThese properties make enzymes attractive biocatalysts for a wide range of industrial and\nbiomedical applications. Over the last two decades, directed evolution of enzymes has\ntransformed the field of protein engineering. Detailed record
	Fluorescence and surface enhanced Raman spectroscopy in microfluidics for monitoring of enzymatic reactions Pilát, Zdeněk ; Šmatlo, Filip ; Ježek, Jan ; Krátký, Stanislav ; Zemánek, Pavel We have implemented two different systems for detecting the concentration of molecules in microfluidic systems. The first method uses optical fibers and detects the intensity of fluorescence, while the second method is using surface enhanced Raman spectroscopy (SERS). Detailed record
	Optically trapped tunable droplet microlasers from liquid crystals Pilát, Zdeněk ; Ježek, Jan ; Jonáš, A. ; Aas, M. ; Kiraz, A. ; Brzobohatý, Oto ; Zemánek, Pavel We created a system for concurrent optical trapping, laser excitation and spectroscopy of liquid crystal droplets doped with a fluorescent dye. We observed whispering gallery modes and lasing of the droplet. We tuned the droplet laser frequency with optical forces, electric current or heating. Detailed record

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